Coding

Part:BBa_K4404000

Designed by: Alexander Rosenberger   Group: iGEM22_Goettingen   (2022-09-30)


acsB1 from A. woodii

This region codes for the acetyl-CoA synthase in the acetogenic bacterium Acetobacterium woodii. To be more precise it codes for the acetyl-CoA synthase subunit (ACS) of the CODH/ACS-complex which catalyzes the formation of acetyl-CoA from CO and [CH3]-Co-FeS-P. This enzyme is crucial for the complementation of the Wood-Ljungdahl metabolic pathway. The gene coding fragment is flanked by specific restriction sites and overhangs. Upstream it is flanked by a Xho1 restriction site and a 5 base pairs overhang. Downstream it is flanked by a Nhe1 restriction site and a 5 base pairs overhang.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1500
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1500
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2133
    Illegal NheI site found at 2187
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1500
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2133
    Illegal BamHI site found at 2171
    Illegal XhoI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1500
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1500
    Illegal EcoRI site found at 1845
    Illegal EcoRI site found at 2133
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None